Meet Dr. Premila Leiphrakpam
About me: I am a Postdoctoral researcher in the Department of Surgery-Acute Care Surgery, College of Medicine, University of Nebraska Medical Center (UNMC). I received my Ph.D. degree from UNMC in 2017. During my doctoral program my research was focused on the identification of potential signaling molecules and pathways involved in cell survival and apoptosis related to human diseases, and I was awarded program of excellence fellowship for my thesis project. In 2019, I was recruited by Dr. Keely Buesing for my expertise in basic and translational research.
My Research: Our lab has been focused on understanding the pathophysiology and molecular mechanism of wood smoke exposure-related acute lung injury, and the effect of oxygen microbubble (OMB) treatment. Since 2014, our lab has been collaborating with external researchers to investigate the therapeutic potential of OMB therapy in small and large animal models of smoke inhalation and intra-tracheal lipopolysaccharide (LPS) induced acute lung injury. My main responsibility is to establish in-vitro research projects in the lab, and my research background in both basic and translational sciences provided me the necessary expertise. We are employing proteomic and genetic approaches to identify potential molecules differentially expressed in lung tissues samples after smoke inhalation and with OMB treatment. With proteomic analysis of lung tissue samples, we have seen promising preliminary results of partial reversal of wood smoke-mediated differential global protein expression with OMB treatment.
How the Mass Spec Core Helped My research: We utilized resources available in the Mass Spectrometry & Proteomic Core Facility Core for our analyses to determine differential global protein expression in wood smoke inhalation induced lung injury and the potential of reversing these protein expressions with OMB treatment. TMT method was utilized to label our pig lung tissue samples to directly compare protein abundance between control animals and smoke exposed animals, with and without OMB treatment. These labeled samples are then analyzed by the core using their Orbitrap Fusion Lumos mass spec. Our collaboration with MSPCF have resulted in the submission of an NIH R01 grant and a research manuscript draft is under preparation for publication in a peer reviewed journal. We greatly appreciate MSPCF Core for their fast turnaround time and for providing technical expertise in troubleshooting our experiments and data analysis. We look forward to working with them in our upcoming projects.