Mass Spectrometry & Proteomics Core Facility

*Christmas in July *

 

What's in the Box?



Although this is missing a red shiny ribbon around, we've got some BIG RED news to share!
A new addition to our mass spectrometers - Orbitrap Exploris 480 MS  from Thermo Sci- has arrived and is here to advance and empower proteomics research on our Campus, and wider. We will be offering proteomics services on our brand new Exploris + FAIMS system, very soon! 
With:

  • High resolution and mass accuracy
  • Selectivity and spectral quality
  • intelligence-driven performance
  • power and reliability
 ..and much more! Most importantly, Exploris is market leading mass spectrometer designed to offer THE BEST quantitative performance.


We are beyond excited and will be sharing more news and features, as we continue working on setting everything up for success. 

 

Demonstrating the power of mass spectrometry with the Thermo Scientific Orbitrap Exploris 480 MS

*|END:WEB_VIDEO|*
What can you detect in my sample?

-Everything that has a mass! That being said, we give you back the list of all the proteins that we found in your sample.


I am only interested in one specific protein.

-In that case, we usually recommend some sort of enrichment, as we can see your protein better if it is not standing in a large crowd.


Can I see post-translational modifications of proteins, and which one?

-Yes, all post-translational modification may be detected. Some of them are tricky however, and you might need to do some specific protein enrichment.

Can I quantify my proteins?

- We can calculate the ratios in expression of all the proteins in your sample. We might need to tag the peptides prior the analysis, but other ways are possible, too. You should use a statistically valid method to find out what in your sample is significant, or eliminate everything that is not up/down-regulated by at least 2 fold (replicates, replicates, replicates).

 





I'VE GOT MY PROTEIN LIST...NOW WHAT?






It is not so complicated if the list is rather short, right? Protein is THERE /protein is NOT THERE.
But what if the protein list is looooong, let's say above 2000 protein IDs? And there is a bunch of stuff you don't really care about.

Here is where you can start, it's like a free 'starter pack' :



1. PANTHER
      is the great free ontology tool, where you can upload your protein list (gene ID) and analyze pathways and biological processes!
Link:





2. REACTOME
    is another great, free, curated and peer-reviewed pathway database. The Reactome website is designed to literally give the user a graphical map of known biological processes and pathways that is also an interface which the user can ‘click through’ to authoritative detailed information on components and their relations. All you have to do is upload your protein ID list.
Link:

 
Amino acids and their name origin

FIND US AT:
DRC I 1044, 1046, 1050


























CONTACT US AT:
mspcf@unmc.edu

Tip Of The Month

We know that you probably like autoclaving, but we don't! We also do not like (sorry!!) detergents, salts, keratins, polymers. Keep it cool...we also don't like high temperatures!

University of Nebraska Medical Center